Fig. 3

Transient overexpression of Nb-CAR7 inhibited TMV RNA accumulation in N. benthamiana plants. a Schematic structure of Nb-CAR7 overexpression vector, 35S-NbCAR7-GFP. b The relative expression level of Nb-CAR7 was increased in plant leaves at 3 days post infiltration with 35S-NbCAR7-GFP vector, using plants infiltrated with 35S-GFP vector as controls. c After TMV inoculation of the plants pre-infiltrated with 35S-NbCAR7-GFP vector, the relative expression level of TMV RNA at 7 dpi was decreased in the TMV-inoculated leaves but increased in the upper leaves. d After TMV inoculation of the plants pre-infiltrated with 35S-NbCAR7-GFP vector, the relative expression level of Nb-CAR7 in the TMV-inoculated leaves was higher than that of control plants at 7 dpi, whereas was lower in the upper leaves than that of control plants. * represents P < 0.05 compared with control group