Fig. 1

Detection of tomato brown rugose fruit virus (ToBRFV) from diseased tomato plants. a Symptoms observed on ToBRFV-infected tomato plants in Yunnan Province of China. b Detection of ToBRFV by RT-PCR with two sets of ToBRFV-specific primers. Lane M, DL2000 DNA marker (TaKaRa). Lanes 1–7 indicate PCR products amplified from seven samples (Y2020–1, Y2020–2, Y2020–3, Y2020–4, Y2020–5, Y2020–6, and Y2020–7), respectively. Lane 8, double distilled water (ddH2O) was used as the negative control