Fig. 5

Protoplast fluorescence images and Immunoblotting (IB) analyses showing differences in subcellular localization of the TALEs between the WT (Nipponbare) and OsIMPα1b-ED#6 plants. a Laser scanning confocal microscopy (LSCM) images of the plant protoplasts at 16 h after transformation with the pthXo1-RFP fusion gene. Each image is representative of 200–300 protoplasts. b IB of proteins extracted from protoplasts equivalent to those in a. c LSCM images of the plant protoplasts at 16 h after transformation with the TALI-RFP fusion gene. In a and c, each image is representative of 200–300 protoplasts; scale bars = 10 µm. d IB of proteins extracted from protoplasts equivalent to those in c. The asterisks and vertical lines indicate unidentified cross-reactive proteins