Fig. 2

Co-inhabitation of Mesorhizobium huakuii strain 7653R-mCherry and Plasmodiophora brassica in cortex cells of rapeseed root. a Strain 7653R-mCherry detected in root hair and cortex cells. Co-inoculated seedlings were grown in a growth chamber for 5–8 days. Bar = 50 µm. b M. huakuii and P. brassicae co-inhabit in cortical cells where resting spores (rs) have developed. Co-inoculated seedlings were grown in a growth chamber for 30 days. The 25 µm-thick sections were sliced using a freezing microtome (Lecica CM1950, Germany). Bar = 20 µm. c Partically enlarged images of b. The corresponding positions are presented as (i) to (V). Bar = 10 µm. d The free-lifestyle strain 7653R-mCherry. Images in b and c were stacked. The red fluorescence was observed under a confocal microscope (Olympus, FV1000). Bar = 10 µm