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Fig. 6 | Phytopathology Research

Fig. 6

From: The regulatory subunit MoB56 of PP2A phosphatase regulates pathogenicity, growth and development in a protein complex with the atypical catalytic subunit Ppg1 in the rice blast fungus Magnaporthe oryzae

Fig. 6

Interaction assay of MoB56 with the scaffold subunit A and catalytic subunit Ppg1. a Yeast two-hybrid analysis of the interaction between regulatory subunit MoB56, catalytic subunit Ppg1 and scaffold subunit PP2Aa. MoB56, Ppg1 and PP2Aa were inserted into vector pGADT7 and/or pGBKT7. BD-MoB56 and AD-Ppg1, BD-Ppg1 and AD-MoB56, BD-Ppg1 and AD-PP2Aa, BD-MoB56 and AD-PP2Aa were co-introduced into yeast Y2HGold strain, respectively, and then incubated on SD-Leu-Trp (as control) and SD-Leu-Trp-His (for selection) for 3–5 days. b Partial proteins precipitated with Ppg1-3Flag and identified by LC-MS/MS. c Co-immunoprecipitation assay shows that MoB56-3HA interacts with Ppg1-3Flag. A nuclear protein (predicted 85 kDa) fused 3Flag tag protein construct was used as a control

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Phytopathology Research

ISSN: 2524-4167