Fig. 6
From: Biocontrol and molecular characterization of Bacillus velezensis D against tobacco bacterial wilt
Effects of crude extracts on cell viability of R. solanacearum. Cell viability of R. solanacearum was detected with PI and SYTO9 after treatment with sterile water (a) or 150 μg/mL crude extracts produced by strain D (b). Live bacterial cells with intact membranes showed green fluorescence, whereas cells with damaged membranes showed red fluorescence. Bars = 20 µm. c The percentage of live cells supplemented with crude extracts after incubation for 15 min. Sterile water was used as a control. Data are presented as the mean ± SD based on three biological replicates; P-values were determined by LSD post-hoc test. Different letters represent significant difference