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Fig. 5 | Phytopathology Research

Fig. 5

From: The sucrose non-fermenting-1 kinase Snf1 is involved in fludioxonil resistance via interacting with the high osmolarity glycerol MAPK kinase Hog1 in Fusarium

Fig. 5

Protein kinase FgSnf1 interacts with FgHog1 and negatively regulates phosphorylation of FgHog1. a The phosphorylation of FgHog1 was detected by immunoblotting in PH-1 and ΔFgSNF1, treated with or without fludioxonil. GAPDH was used as a loading control. b Quantification of phosphorylation levels of FgHog1 in a. c Sub-cellular localization of FgHog1-GFP in PH-1 and ΔFgSNF1, strains were treated with fludioxonil at 1 μg/mL or DMSO for 5 min. d Interaction between FgSnf1 and FgHog1 was verified by yeast two- hybrid assays. e Interaction between FgSnf1 and FgHog1 was verified by Co-IP assays

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Phytopathology Research

ISSN: 2524-4167