Fig. 3
From: Overexpression of wheat spermidine synthase gene enhances wheat resistance to Fusarium head blight
Overexpression of TaSPDS-7D1 in wheat confers resistance to FHB. a PCR detection of the wild type (WT) and TaSPDS-7D1-overexpressing lines (OE3 and OE21). M, DL2000 DNA marker. b Relative expression levels of TaSPDS-7D1 in WT and TaSPDS-7D1-overexpressing lines. The error bar indicates standard deviation (SD) from three independent replicates (n = 3). c Western blot of the WT and TaSPDS-7D1-overexpressing lines using an anti-HA antibody. d Representative images of the WT and TaSPDS-7D1-overexpressing lines with F. graminearum inoculation. e Diseased spikelet number of the WT and TaSPDS-7D1-overexpressing lines with F. graminearum inoculation. f DON levels of WT and TaSPDS-7D1-overexpressing lines. The error bar indicates standard errors of the means (n varies for each column and is shown in each case directly on the graphs). P-values are from the Student’s t-tests