Fig. 5

MoSey1 regulates ER stress in M. oryzae. a MoSey1-GFP co-localizes with the ER marker RFP-MoLhs1 in conidia (CO), hyphae (HY), appressoria (AP), and invasive hyphae (IH). The strain co-expressing Sey1-GFP and RFP-MoLhs1 was visualized under a confocal microscope. Scale bars: 10 μm. b, c Vegetative growths and inhibition rates of the indicated strains on media supplemented with ER stress-inducing agents. d RT-PCR analysis for the UPR-induced splicing in different fungal strains. e Transcription levels of genes regulating the UPR pathway in M. oryzae. Values are presented as means ± SD, calculated from three independent experiments. Double asterisks denote significant differences at P < 0.01