Fig. 7
Involvement of MoSey1 in autophagy. a, b Localization of GFP-MoAtg8 in ΔMosey1 mutant and wild type strains. The mycelia were cultured under 28℃ for one day in CM liquid media, which then were washed with distilled water and transferred to MM-N liquid media containing 2 mM PMSF for 4 h. CMAC was used to stain the mycelia. Scale bars: 5 μm. c Observation of organelles and autophagic bodies in hyphae cultured in MM-N for 4 h. Scale bars: 1 μm. The arrow indicates an autophagic body. d Total mycelial proteins were extracted and analyzed after autophagy induction. The proteins were detected by western blot analysis with anti-GFP and anti-Actin antibodies. The flux of autophagy was estimated by calculating the amount of free GFP to GFP-Atg8. The band intensity was quantified by Image J software