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Fig. 2 | Phytopathology Research

Fig. 2

From: Host plant-derived benzoic acid interferes with 4-hydroxybenzoic acid degradation in the phytopathogen Xanthomonas campestris by competitively binding to PobR

Fig. 2

Construction and verification of the reporter strain XC1::PpobA-gusA to monitor the transcriptional levels of pobA. a Schematic diagram of the reporter strain for monitoring pobA transcription. b The pobA promoter PpobA-dependent β-glucuronidase (GUS) activity in XYS liquid medium or XYSG agar plates supplemented with 0.001–1.0 mM 4-hydroxybenzoic acid (4-HBA). c The quantitative reverse transcription-PCR (qRT-PCR) analysis of the relative expression level of pobA in the presence of 0.001–0.5 mM 4-HBA. The mean values of three technical repeats are shown with the standard deviation. Statistically significant differences are indicated by one asterisk (p ≤ 0.05), two asterisks (p ≤ 0.01), or three asterisks (p ≤ 0.001)

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Phytopathology Research

ISSN: 2524-4167