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Fig. 3 | Phytopathology Research

Fig. 3

From: Host plant-derived benzoic acid interferes with 4-hydroxybenzoic acid degradation in the phytopathogen Xanthomonas campestris by competitively binding to PobR

Fig. 3

Effects of 4-HBA-like analogues on PpobA-dependent GUS activity in presence of 4-HBA. a GUS activities of the XC1::PpobA-gusA cultures in presence of 0.2 mM 4-HBA and 4-HBA analogues (0.1–0.4 mM). b Left, colonies of strain XC1::PpobA-gusA on agar plates supplemented with 4-HBA and BA; Right, quantitative reverse transcription-PCR (qRT-PCR) analysis of the relative expression level of pobA in 4-HBA treated XC1 in the presence of 0.1–0.2 mM BA. c Relative β-glucuronidase (GUS) activities of the XC1::PpobA-gusA cultures in the presence of five combinations of low levels of 4-HBA and BA . 4-HCA, 4-hydroxycinnamic acid; NaSA, sodium salicylate; SA, 2-hydroxybenzoic acid; 3-HBA, 3-hydroxybenzoic acid; 3M4HBA, 4-hydroxy-3-methoxybenzoic acid; 3M4HBD, 4-hydroxy-3-methoxybenzaldehyde; 3,4-DHBA, protocatechuic acid; 3,4,5-THBA, 3,4,5-trihydroxybenzoic acid; 3MBA, 3-methoxybenzoic acid; CA, cinnamic acid; 3-HCA, 3-hydroxycinnamic acid; 3,4-DHCA, caffeic acid; BA, benzoic acid. The mean values of three technical repeats are shown with the standard deviation. Statistically significant differences are indicated by one asterisk (p ≤ 0.05) or two asterisks (p ≤ 0.01)

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Phytopathology Research

ISSN: 2524-4167