Fig. 2

Identification the relationship of FvCtf1α with other transcription factors CTFs. a The localizations of CTF transcription factor FvFarA-GFP and FvFarB-GFP were observed in the nucleus with the nucleus stained by DAPI, respectively. b The interaction of FvCtf1 with FvFarA or FvFarB analyses by BiFC fluorescence. Five plasmid pairs were co-transformed into wild-type Fv7600 protoplast, with three plasmid pairs FvCtf1α-NYFP and CYFP, NYFP and FvFarA-CYFP, NYFP and FvFarB-CYFP using as negative controls, while the plasmid pairs FvCtf1α-NYFP and FvFarA-CYFP, FvCtf1α- NYFP, and FvFarB-CYFP were used as tests. BiFC fluorescence images were captured on confocal laser scanning microscope. Scale bars: 10 µm