Fig. 3

Defect on carbon metabolic, producing conidia and cell wall degrading enzyme activity of ΔFvctf1α deleted mutants. a To compare the nutrient utilization capacity of the WT and mutant strains, the vegetative growths of the strains were monitored on MM with short chain carbon, respectively. b The vegetative growths of the strains were monitored on MM with long chain carbon, respectively. c, d The colony diameters of the cultures were measured and inhibition of mycelial growth analyzed by t-test on short chain carbon, on long chain carbon. e The expression levels of the carbon metabolic related genes were significantly down-regulated in ΔFvctf1α by qRT-PCR with the reference gene TUB2 using the 2^−ΔΔCt method. f Measurement and statistical comparison of conidia from WT and mutant strains after inoculation on CMII at 28°C for 3 days. Error bars denote standard deviations from three repeated experiments and asterisks represent a significant difference. g The activity of three cell wall degrading enzyme including cutinase, pectatinase, and celluase were assessed in ΔFvctf1α and WT, respectively. The experiment was performed three times with similar results and error bars represent the standard deviation and asterisks represent a significant difference (t-test, *: p < 0.05,**: p < 0.01)