Fig. 5

FvCtf1α plays key role in fumonisin B1 (FB1) production. a FB1 levels in the samples were measured using the formula FB1/TUB2 DNA. Surface sterilized B73 corn kernels were inoculated with the WT and mutant conidia suspensions and incubated for 10 d. FB1 levels were quantified using ELISA Kit. F. verticillioides biomass was quantified by measuring F. verticillioides TUB2 DNA in samples. ELISA: enzyme-linked immunosorbent assay. b Relative FUM genes expression were compared by ΔFvctf1α and WT, respectively. qRT-PCR was used to quantify transcript level of FUM genes to the reference gene TUB2 using the 2^−ΔΔCt method. The experiment was performed three times. Error bars represent the standard deviation. (t-test, *: p < 0.05, **: p < 0.01). c FvCtf1α can bind the promoters of the five FUM genes FvFUM1, FvFUM2, FvFUM6, FvFUM14, and FvFUM16 by yeast one-hybrid, respectively. pAbAi::FvFUM1pro, pAbAi::FvFUM2pro, pAbAi::FvFUM6pro, pAbAi::FvFUM14pro, pAbAi::FvFUM16pro vectors, and pGADT7-FvCtf1α were constructed. The Y1H-Gold strain was sequentially transferred into two vectors, first transferred into the pAbAi::FvFUM1pro vector (or pAbAi::FvFUM2pro vector), selecting with SD/-Ura medium, and reintroduced into the pGADT7-FvCtf1α vector, selecting with SD/-Ura media with 0, 100, 150, 200 ng/mL Aureobasidin A (AbA). The p53-AbAi vector transformants were used as the negative control and pAbAi::pro vector transformants as the positive control. d FvCtf1α can bind the promoters of the five FUM genes at CAMCA site by Multiple EM for Motif Elicitation (MEME) software