Fig. 2

Efficient gene disruptions with the Mo_tRNAArg-gRNA-Cas9/pKOV21 system in P. oryzae. a The loci of selected genes for assessing the efficiency of the Mo_tRNAArg-gRNA-Cas9/pKOV21 system in P. oryzae. Six selected genes are located in different chromosomes, and Bip2 is quite close to the centromere on chromosome 4. Scale bars = 1 Mbp. b The gene replacement rates of three reported genes with the Mo_tRNAArg-gRNA-Cas9/pKOV21 system