Fig. 1

Effects of CHV1 infection on the development and pathogenicity of F. graminearum. a, c, and e Infection assays were conducted on tomato fruits, coleoptiles, and wheat heads, using virus-free and CHV1-infected F. graminearum PH-1 (PH-1). Disease symptoms were photographed at 7 days and 14 days post inoculation (dpi). b, d, and f The lesion area of tomato fruits, lesion length of coleoptiles, and FHB disease progression were measured. g The impact of CHV1 infection on conidia was assessed. h CHV1 infection reduced the number of conidia but did not affect conidia morphology and germination after 6 hours of incubation. i and j The formation of perithecia and asci/ascospores was observed in virus-free and CHV1-infected strains after 15 days of culture in carrot medium at 25 and the number of perithecia was calculated. The data are expressed as mean ± SD (n=3 or n=10). ** indicates a significant difference at p<0.01, and *** indicates a significant difference at p<0.001 (Student’s t-test). Scale bars: 20 μm