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Fig. 3 | Phytopathology Research

Fig. 3

From: Cryphonectria hypovirus 1 infection suppresses the pathogenicity but increases the mycotoxin deoxynivalenol production of Fusarium graminearum

Fig. 3

CHV1 infection increases the biosynthesis of DON. a DON production was determined in virus-free and CHV1-infected strains grown in TBI, rice medium, and wheat spikes. b The formation of toxisomes in the mycelia of virus-free and CHV1-infected Tri1-GFP strains grown in TBI for 48 hours was observed. The Tri1-GFP strain carries a reporter construct that expresses Tri1 fused with green fluorescence protein (GFP) under its native promoter. GFP fluorescence was visualized using confocal laser scanning microscopy (CLSM). c The total proteins were extracted from the mycelia and subjected to western blotting analysis using GFP-specific antibodies. d The total RNA was extracted from the mycelia and the transcript expression of Tri1-GFP was analyzed by qRT-PCR. e, f The transcript expression of key genes (Tri1, Tri4, Tri5, and Tri6) involved in the biosynthesis of DON was analyzed. The total ssRNA was isolated from the mycelia of virus-free and CHV1-infected strains grown in PDA or TBI, and qRT-PCR analysis was carried out. The data are presented as mean ± SD (n=3). ns indicates no differences, * indicates significant differences at p<0.05, ** indicates a significant difference at p<0.01, *** indicates significant differences at p<0.001 (Student’s t-test)

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Phytopathology Research

ISSN: 2524-4167