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Fig. 5 | Phytopathology Research

Fig. 5

From: Cryphonectria hypovirus 1 infection suppresses the pathogenicity but increases the mycotoxin deoxynivalenol production of Fusarium graminearum

Fig. 5

CHV1 reduces the quantity of lipid droplet (LD) and inhibiting LD biosynthesis enhances CHV1 replication. a The subcellular distribution of LDs in the hyphal cells of F. graminearum was examined using BODIPY staining (green fluorescence). The mycelia were cultured in PDB, PDB containing 5 μM/mL Triacsin C, and mycelia of the CHV1-infected strain were cultured in PDB medium. Confocal laser scanning microscopy was used to observe LDs. b The number of LDs in hyphal cells was measured in an area of 81 μm2. Data are presented as mean ± SD (n=10). c The accumulation of CHV1 in fungal strains was analyzed by qRT-PCR. The total ssRNA was extracted from the mycelia of CHV1-infected strains cultured in PDB and PDB Triacsin C (5 μM/mL) and subjected to qRT-PCR analysis. The data are presented as mean ± SD (n=3). The different letters indicate a significant difference at p<0.05 (one-way ANOVA). ** indicates a significant difference at p<0.01 (Student’s t-test)

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Phytopathology Research

ISSN: 2524-4167