Fig. 6

CHV1 reduced the transcription of FgNem1/FgCak1/FgSpo7 or FgPah1 and decreased LD formation. a Both virus-free and CHV1-infected strains of F. graminearum cultured in TBI medium. The total ssRNA was isolated from the mycelia and analyzed using qRT-PCR. The results are displayed as mean ± SD (n=3). b, c BODIPY staining (green fluorescence) was employed to investigate the LD formation in the hyphal cells of F. graminearum. LD observation was performed using confocal laser scanning microscopy, and the quantification of LDs in hyphal cells within an 81μm2 area is presented, as mean ± SD (n=10). *** indicates a significant difference at p < 0.001 (Student’s t-test)