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Table 1 Transformation efficiency of GLB191

From: Efficient transformation and genome editing in a nondomesticated, biocontrol strain, Bacillus subtilis GLB191

Plasmids

Natural transformation

Electroporation

−

+

Integrative plasmid pDG1662

0.00 ± 0.00 b

10.00 ± 0.82 a

9.67 ± 0.94 a

Replicative plasmid pHT01

0.00 ± 0.00 b

22.00 ± 6.68 a

20.67 ± 1.70 a

  1. Transformants per µg of DNA
  2. − indicates not overexpression of comK and comS, + indicates overexpression of comK and comS in pWYB108
  3. Different letters on the same row indicate significant differences between different treatments according to the least significant difference (LSD) test (p < 0.05)
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Phytopathology Research

ISSN: 2524-4167