Efficient transformation and genome editing in a nondomesticated, biocontrol strain, Bacillus subtilis GLB191

Phytopathology Research

Table 3 ANOVA of the regression model obtained from the Box–Behnken experimental design data conducted to assess the impact of significant factors on the electroporation efficiency of GLB191

Source	Sum of squares	df	Mean square	F-value	P-value	Significance^a
Model	4781.43	14	341.53	13.87	< 0.0001	**
A (glycine)	114.08	1	114.08	4.63	0.0493	*
B (DL-threonine)	1833.56	1	1833.56	74.46	< 0.0001	**
C (Tween 80)	124.59	1	124.59	5.06	0.0411	*
D (voltage)	464.59	1	464.59	18.87	0.0007	**
AB	2.25	1	2.25	0.0914	0.7669
AC	0.1111	1	0.1111	0.0045	0.9474
AD	13.44	1	13.44	0.5459	0.4722
BC	21.78	1	21.78	0.8843	0.363
BD	25	1	25	1.02	0.3308
CD	0.1111	1	0.1111	0.0045	0.9474
A²	442.08	1	442.08	17.95	0.0008	**
B²	516.36	1	516.36	20.97	0.0004	**
C²	77.83	1	77.83	3.16	0.0972
D²	1830.14	1	1830.14	74.32	< 0.0001	**
Residual	344.76	14	24.63
Lack of fit	263.34	10	26.33	1.29	0.433
Pure error	81.42	4	20.36
Cor total	5126.2	28

^a * and ** indicate statistical significance at p < 0.05 and p < 0.01, respectively