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Table 4 Optimized conditions to obtain maximum transformation efficiency

From: Efficient transformation and genome editing in a nondomesticated, biocontrol strain, Bacillus subtilis GLB191

Glycine (M)

DLthreonine (M)

Tween 80 (%)

Voltage (KV)

Predicted transformation efficiency (CFU/µg of DNA)

Desirability

Experimental transformation efficiency (CFU/µg of DNA)

0.42

0.28

0.03

2.25

76.54

0.93

74.00 ± 5.10

  1. Different letters in the same row indicate significant differences between different treatments according to the least significant difference (LSD) test (p < 0.05)
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Phytopathology Research

ISSN: 2524-4167