Fig. 1

Southern blot patterns depicting the 11 genotypes of Pakistani Xoo isolates. Genomic DNAs from the mentioned strains underwent BamHI digestion and were subsequently hybridized with an internal SphI fragment of PthXo1. The 101 strains were categorized into 11 distinct genotypes: G1 (24 strains), G2 (2 strains), G3 (51 strains), G4 (17 strains), and G5 to G11, each containing a single strain, respectively (Table 1). Genotypes are displayed at the top of the image beneath the strain names. The hybridizing bands of varying sizes are identified by different letters (A to T) on the right side of the figure, while bands marked with an asterisk (*) were unique to a single strain (pointed out with the red arrows in the figure). TALE fragments D = 2, E = 2, L = 4 (in all genotypes); O = 2 (G1 to G6, G8, G10, and G11), Q = 2 (G7 to G11), and R = 2 (G8, G10, and G11) are intensely hybridizing and were considered multiple (Table 1). Strain names were assigned using a letter representing their province of origin, followed by a number, except for LD (Lower Dir, KP), BG (Battagram, KP), and BN (Bannu, KP) strains. The province abbreviations used are as follows: P for Punjab and KP for Khyber Pakhtunkhwa. The left lanes of the image feature the λEcoT14 marker (base pairs; bp) and strain LN18 for reference. The right lanes represent the PH strain (PXO99^A tal-free) containing PthXo1, PthXo2, and AvrXa7 in trans that were used to highlight the major virulence tal genes