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Table 3 Pathogenicity assay of Pakistani Xoo genotypes in susceptibility genes edited and different R-genes near-isogenic rice lines

From: TALome and phenotypic analysis of Pakistani Xanthomonas oryzae pv. oryzae population revealed novel virulent TALEs contributing to bacterial blight of rice

Strain/genotype

Kitaake NILs accommodating S genes mutated EBEs

IR24/Kitaake NILs containing different R genes

Kitaake

MS1K

MS3K

MS4K

MS13K

MS14K

MS34K

MS134K

IR24

IRBB3

IRBB4

IRBB5

CBB23

Kit-Xa1

PXO99A

S

R

S

S

R

R

S

R

S

S

S

S

R

S

LN18

S

S

S

S

S

S

R

R

S

S

S

R

R

R

PXO86

S

S

S

R

S

R

R

R

S

R

S

R

R

S

P-10

S

R

S

S

R

R

S

R

S

S

S

R

R

S

P-14

R

R

R

R

R

R

R

R

R

R

R

R

R

R

P-18

S

S

S

R

S

R

R

R

S

S

S

R

R

S

P-27

R

R

R

R

R

R

R

R

R

R

R

R

R

R

P-35

R

R

R

R

R

R

R

R

R

R

R

R

R

R

KP-3

S

MR

S

S

MR

MR

S

R

S

S

S

MS

R

S

KP-22

S

MS

S

S

MS

MS

S

MS

S

S

MR

R

R

S

KP-24

S

R

S

S

MR

MR

S

R

S

S

S

S

R

S

LD-5

S

MS

S

S

MS

MS

S

MS

S

S

MS

MR

R

S

LD-13

S

MR

S

S

MR

MR

S

R

S

S

S

MS

R

S

BG-3

S

R

S

S

R

R

S

R

S

S

S

S

R

S

  1. Reactions of cv. Kitaake and Kitaake with edited OsSWEET genes EBE (MS1K, mutation in OsSWEET11a; MS3K, mutation in OsSWEET13; MS4K, mutation in OsSWEET14 and so on in double and triple combinations); Kit-Xa1 (Kitaake with Xa1 gene), and near-isogenic lines (NIL) carrying different R-genes (IR24, IRBB3, IRBB4, IRBB5, and IRBB23) against Pakistani Xoo genotypes. The well-characterized strains from Philippines (PXO99A and PXO86) and China (LN18) were included for reference. R, resistant (lesion length ≤ 3.0 cm); MR, moderately resistant (lesion length 3.0–5.0 cm); MS, moderately susceptible (lesion length 5.0–8.0 cm) and S, susceptible (lesion length ≥ 8.0 cm). Xoo strains/genotypes KP-22 and LD-5 show compatibility with cv. Kitaake and its EBE defective rice lines (italic highlighted), whereas P-14, P-27, and P-35 represent non-compatible genotypes to all cv. Kitaake and IR24 NILs (bold highlighted). The experiment was repeated and produced similar results