Fig. 2

Construction and validation of infectious clone of SCYLV in N. benthamiana. a Schematic representation of the constructing strategy for the full-length cDNA clone of SCYLV. b RT-PCR detection of stalks of N. benthamiana infected by Agroinoculation with the infectious clone of pCB301-SCYLV at 8dpi. SCYLV detection was performed using RT-PCR with SCYLV-specific primers (SCYLV-TF and SCYLV-TR). NbACTIN as a N. benthamiana reference gene. c Symptoms of N. benthamiana at 8, 16, and 26 days after inoculation with pCB301 or pCB301-SCYLV by Agroinoculation. Bars = 1 cm