Fig. 5
From: Development and application of sugarcane yellow leaf virus vectors in sugarcane
Infectivity of SCYLV and SCYLV-EGFP infectious clone in sugarcane and assessment of sugarcane resistance of SCYLV. a SCYLV detection was performed using RT-PCR with SCYLV-specific primers (SCYLV-TF and SCYLV-TR). b Infectivity of SCYLV-EGFP in Badila observed under white light (left panel) and UV light (right panel) at 40 dpi. Bars = 1 cm. c RT-PCR detection of SCYLV in Badila systemic leaves by EGFP-TF and SCYLV-2R. d Detection of SCYLV-EGFP by Western blot using antibody against EGFP at 40 dpi in sugarcane plants. Badila plants inoculated with empty vector or SCYLV-EGFP, respectively. NS represents non-specific binding. e Detection of the SCYLV infected LC05136 cultivar or GT10-2118 cultivar by RT-PCR. ScGADPH as a sugarcane reference gene