Fig. 7

Monitoring of mitophagy using CaMts-GFP. a The localization of CaMts-GFP was observed under nutrient-rich (PDB) and nitrogen-starved (SD-N) conditions.s The specified strain was grown in the PDB medium for 2 days, followed by transfer to an SD-N medium for 12 h. The vacuoles were stained with CAMC (7-amino-4-chloromethylcoumarin) prior to observation using a confocal laser scanning microscope. Scale bar, 5 μm. b CaMts-GFP degradation was observed under nitrogen starvation conditions using Western blot assay with an anti-GFP antibody. GAPDH served as an internal reference