Fig. 4

ClRPSA-2 negatively regulates CP expression. a Western blotting (WB) analysis was used to detect ClRPSA-2 expression at 3 days post infiltration (dpi). An anti-GFP antibody was used. b The relative expression level of ClRPSA-2 was detected by real-time quantitative PCR analysis (RT-qPCR). Actin was an internal reference gene, t-test, data are mean ± SD, **** P < 0.0001. c, d RT-qPCR and WB analysis were used to detect CP expression. Anti-CYVCV CP antibody was used for WB analysis; Actin was an internal reference gene for RT-qPCR analysis, t-test, data are mean ± SD, n = 9, *** P < 0.001, **** P < 0.0001. e WB analysis detected ClRPSA-2 segments expression. f, g RT-qPCR and WB analysis detected CP expression in ClRPSA-2 segments expression Eureka lemon leaves. Actin was an internal reference gene, one-way ANOVA test at 0.05 level (n = 9), data are mean ± SD; An anti-CYVCV CP antibody was used for WB analysis