Fig. 5

ClRPSA-2 triggers jasmonic acid (JA) and photosynthetic pathway expression. a, b Relative expression level of JA- and photosynthesis-related genes in ClRPSA-2 transiently expressed Eureka lemon leaves. Actin was an internal reference gene, t-test, data are mean ± SD, n = 9, ** P < 0.01, *** P < 0.001, **** P < 0.0001. c, d The JA content and F_v/F_m in the ClRPSA-2 transient expressed and silenced Eureka lemon leaves. t-test, data are mean ± SD, n = 5, * P < 0.05, *** P < 0.001, **** P < 0.0001. e, f The JA content and F_v/F_m in ClRPSA-2 and ClRPSA-2 + CP transiently expressed Eureka lemon leaves. T-test, data are mean ± SD, n = 5, *P < 0.05, ***P < 0.001. g, h Relative expression level of JA- and photosynthesis-related genes in ClRPSA-2 and ClRPSA-2 + CP transiently expressed Eureka lemon leaves. Actin was an internal reference gene, t-test, data are mean ± SD, n = 9, ** P < 0.01, *** P < 0.001, **** P < 0.0001. i Relative expression level of CP in CP and ClRPSA-2 + CP transiently expressed Eureka lemon leaves. Actin was an internal reference gene, t-test, data are mean ± SD, n = 9, **** P < 0.0001. j Western blotting was used to detect the accumulation of CP with anti-CYVCV CP antibody