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Fig. 3 | Phytopathology Research

Fig. 3

From: Rapid diagnosis of Diaporthe helianthi in sunflower using RPA/CRISPR-Cas12 and lateral flow assay

Fig. 3

Optimization of RPA/CRISPR-Cas12a detection method. a Fluorescence intensity of RPA products with different RPA reaction time. W1–W5 are the corresponding negative controls. b Fluorescence intensity with different FQ reporter concentrations in the CRISPR/Cas12a reaction system. W1-W5 are the corresponding negative controls. c The lateral flow strips of CRISPR/Cas12a reaction products with different reaction time. d The lateral flow strips of CRISPR/Cas12a reaction products with different LF reporter concentrations. T, test line; C, control line

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Phytopathology Research

ISSN: 2524-4167