Fig. 2

Requirements of N, P, L, and VSR proteins for agMR/gMR reporter gene expression. a GFP foci observation under ultraviolet light (UV). GFP foci in agroinfiltrated leaf tissues were photographed at 12 dpi. Scale bar = 5 cm. b, c Fluorescent microscope images showing the GFP and RFP foci in infiltrated leaves at 12 dpi. Scale bar = 20 μm. d, e Western blot analysis showing accumulation of the GFP and RFP proteins in the leaves shown in panels b and c with mouse antibodies against GFP and RFP. The mock sample was infiltrated with Agrobacterium harboring the empty pGD vector. The mouse antibody against actin was used for protein loading controls