Fig. 3

RSMV GFP-P informs VF-like granules and is functional for agMR replication. a Schematic diagram of pGD vectors for the expression of P-GFP and GFP-P. b Subcellular distribution of P-GFP and GFP-P in agroinfiltrated leaves of H2B-RFP transgenic N. benthamiana at 55 hpi. Scale bar = 20 μm. c Immunosorbent electron microscopy analysis of P subcellular localization patterns in non-infected and RSMV-infected rice leaves cells at 30 dpi. Bar = 500 nm. V, virion; VF, viral factory. d qRT-PCR analysis of minigenome RNA replication in the agMR with the combination of pGD-P, GFP-P, or P-GFP, respectively. One-way t-test was used for analyses, "**" indicates the highly significant difference between the datas, P < 0.01. e Western blot analysis showing the accumulation of the RFP proteins in the leaves shown in panel (d) with mouse antibodies against RFP. The accumulation of actin protein was used to standardize the concentration of the RFP protein level, and protein concentration further analyzed by ImageJ. f Fluorescent microscope images showing the RFP foci in infiltrated leaves at 6 dpi. Scale bar = 100 μm