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Fig. 1 | Phytopathology Research

Fig. 1

From: Insights into geminiviral pathogenesis: interaction between βC1 protein and GLABROUS1 enhancer binding protein (GeBP) in Solanaceae

Fig. 1

SlGeBP interacts with TbCSB βC1 in vitro and in vivo. a Y2H analysis of the interaction between βC1 and candidate interacting proteins in vivo. The transformed yeast cells were subjected to tenfold serial dilutions and plated on SD/-Trp-Leu-His-Ade medium for 3 days. Yeast cells co-transformed with AD-T and BK-P53 were served as positive controls; yeast cells co-transformed with AD-T and BK-Lam, AD-βC1 and BK, or AD and BK-SlGeBP were used as negative controls. b LCI analysis of the interaction between βC1 and SlGeBP in N. benthamiana leaves. βC1-NLuc co-expressed with CLuc or NLuc co-expressed with CLuc-SlGeBP, and NLuc co-expressed with CLuc, were used as negative controls. c BiFC analysis of the interaction between SlGeBP and βC1 in N. benthamiana leaves. The RFP-H2B is nuclear localization marker. Co-expression of YN-SlGeBP and YC or YN and YC-βC1 were used as negative controls. Confocal imaging was performed at 48 hpai. Scale bars: 10 μm

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Phytopathology Research

ISSN: 2524-4167