Fig. 5

Detection of tomato spotted wilt virus (TSWV) in a Hymenocallis speciosa leaf with systemic infection. a Image of the leaf showing the location of the tissue sampling sites (numbered 1 to 15) relative to the symptoms. b Detection of TSWV by reverse transcription polymerase chain reaction. The lane number corresponds to the sampling site in a. The target amplicon is about 900 bp, and N, P, and TF are the negative, positive, and template-free controls, respectively. N was extracted from a TSWV-negative spider lily sample, and P from a TSWV-positive spider lily sample