Evaluation of spider lilies (Hymenocallis spp.) as sentinel plants for orthotospoviruses

Phytopathology Research

Table 1 Primers used in this study

Primer name^a	Primer sequence	Amplicon size
PCR Primers (5′–3′)
TospoRp-F^b	GGCATCATRTAIATIGCIARRTAIACATT	~ 320 bp
TospoRp-R^b	ATGGGIATITTYGAYTTYATGIGRYATGC
TSWV-N-F1	AGCAAGCACAAGCAATAAAGATAAAGAAA	~ 930 bp
TSWV-N-R1	CGACTGCGGVATACAGAGTTG
CaCV-N-F2	GGGAARATRAYYGGTGCAAG	532 bp
CaCV-N-R1	ACGTTTCCAKAGTAAWCACC
CaCV-N-F1	ACATGAYAATAGATTAATATGAATTGACTTATAATTAAATATAAC	~ 950 bp
CaCV-N-R1	as above
NAD5-F	GATGCTTCTTGGGGCTTCTT	325 bp from plant RNA
NAD5-R	GAGCTRATCCRAAATCMCCTACTCG
CaCV-S-F	AGAGCAATCGRGGCWTCTAATAATTCAAG	~ 3.5 to 4 kb
CaCV-S-R	AGAGCAATCGAGGCRCTAATAAAATC
C1-J-1751^c	GGATCACCTGATATAGCATTYCC	~ 480 bp
C1-N-2191^d	CCCGGTAAAATTAAAATATAAACTTC
Sequencing Primers (5′–3′)
TospoRp-F^b	as above
TospoRp-R^b	as above
TSWV-N-F2	TCRACIGAAGCAATRAGAGGTAA
TSWV-N-R2	ATGACCTYCAGAAGGCTTGA
CaCV-N-F2	as above
CaCV-N-R2	GAYTGGACTTTYAAGAGRACAGA
C1-J-1751^c	as above
C1-N-2191^d	as above
Reverse Transcription Primer (5′–3′)
CaCV-S-RT	AGAGCAATCGAGGCRCTAAT

^aAll primers were designed by the first author unless specifically stated otherwise
^bDesigned by A.D.W. Geering
^cLin et al. 2004
^dSimon et al. 1994