Fig. 1

In situ hybridization and analysis of the developmental expression pattern of MgTTL1. a Localization of MgTTL1 expression in the esophageal gland of pre-parasitic second-stage juveniles of M. graminicola using in situ hybridization. Fixed nematodes were hybridized with sense (left) and antisense (right) DNA probes targeting MgTTL1. Scale bars represent 50 µm. EG, esophageal gland cells; M, metacorpus; S, stylet. b The relative expression levels of MgTTL1 were detected using qRT-PCR in five different developmental stages of M. graminicola (J2, par-J2, par-J3/4, and female). Data shown are the means of three repeats plus standard deviation (SD) (n = 3). Samples were compared with the Tukey-test (*P < 0.05; **P < 0.01). par-J2, par-J3 and par-J4: parasitic second-, third- and fourth-stage juveniles