Fig. 4

The role of mgr-mir-228 in regulating MgTTL1 expression and nematode infection processes. a Pairing schemes of mgr-mir-228 and MgTTL1 mRNA interactions are illustrated. The matching position of MgTTL1 mRNA starts at 3’ UTR. b MgTTL1 is the direct target of mgr-mir-228. pMIR-REPOR-MgTTL1 luciferase constructs containing a wild type (WT) or mutant type (MT) target sequence were transfected into HEK293 cells. Results showed the mean values of four independent experiments ± SD (n = 4). c The expression level of mgr-mir-228 at the J2 stage and pra-J3/J4 stage (5 dpi). Data shown are means ± SD (n = 3). All data were analyzed by ANOVA (one-way) with a Tukey’s test (**P < 0.01)