Fig. 6

MgTTL1 as a negative regulator of ROS-mediated plant immunity. a Detection of ROS generation following flg22 stimulation. Leaf discs were cut from N. benthamiana plants three days after infiltration with A. tumefaciens overexpressing MgTTL1 or GFP (control). b Assessment of soybean cyst nematode (SCN) development past the J2 stage at 10 dpi in soybean roots transiently overexpressing MgTTL1 or empty vector (control). Nematode matured beyond J2 (# > J2)/(total #): the proportion of J3 and J4 in the total nematodes. Scale bars represent 100 µm. c Representative images and luminescence readings of Arabidopsis leaves of transgenic lines overexpressing MgTTL1 (MgTTL1-OE1 and MgTTL1-OE2) compared to the empty vector control (EV). Bar = 1 cm. d Bacterial counts for Arabidopsis transgenic lines overexpressing MgTTL1 or empty vector controls. Statistical analysis was performed using ANOVA (one-way) with a Tukey's test. Significant differences are indicated by asterisks (*P < 0.05; ***P < 0.001)