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Table 3 The data analysis of DAS-ELISA results conducted using two replicates per rice genotype under test, a non-inoculated genotype as control, and four replicates for the PBST control

From: Pathogen dynamism and variability of rice yellow mottle virus in Kenya

Sample/Genotype

OD405nm

ODcor*

Mean ODcor

Conclusion**

PBS-T control

0.039

0.00175

0

 

0.038

0.00075

0.037

 − 0.00025

0.035

 − 0.00225

Non-inoculated control

0.346

0.30875

0.28625

Negative

0.301

0.26375

Komboka

1.15

1.11275

0.99625

Positive

0.917

0.87975

IR 64

1.163

1.12575

1.10575

Positive

1.123

1.08575

AT 054

1.005

0.96775

0.98625

Positive

1.042

1.00475

Basmati 217

0.953

0.91575

0.85625

Positive

0.834

0.79675

Basmati 370 (Pishori)

0.802

0.76475

0.77975

Positive

0.832

0.79475

BW 196

1.31

1.27275

1.20925

Positive

1.183

1.14575

IR2793-80–01

1.473

1.43575

1.42075

Positive

1.443

1.40575

Pwani Gold

1.227

1.18975

1.06475

Positive

0.977

0.93975

Positive control (commercial)

1.454

1.41675

1.36625

Positive

1.353

1.31575

  1. *ODcor corresponds to the OD405nm of the sample minus the mean of OD405nm of the PBST controls
  2. **Samples are considered positive if the mean (ODcor) is greater than 0.1 and twice that of the non-inoculated control (0.5725)