Fig. 3
From: Establishment of sugarcane mosaic virus-based vector for dual gene expression in maize
Stability of the GFP gene insertion in SCMV-based single-gene expression constructs. Total RNA isolated from leaves infected with GFP-expressed SCMV vectors of SCMV-GUSNIb/CP (a) and SCMV-GUSP1/HC−Pro (b) were analyzed at 4, 7, 14, 21, and 28 dpi by RT-PCR amplification with primers flanking the insertion of GFP (upper panels). Plasmids pSCMV and pSCMV-GUSNIb/CP (a) or pSCMV-GUSP1/HC−Pro (b) were amplified as controls. Levels of CP transcript (lower panels) amplified from tested samples were served as internal controls