Fig. 5

Stability of gene insertions in SCMV-based dual-gene expression constructs. Total RNA isolated from leaves infected with SCMV-mCherry^P1/HC−Pro-GFP^NIb/CP (a) or SCMV-GFP^P1/HC−Pro-mCherry^NIb/CP (b) were analyzed at 4, 7, 14, 21, and 28 dpi by RT-PCR amplification with primers flanking the insertion of GFP and mCherry, respectively (upper two panels). Plasmids pSCMV and SCMV-mCherry^P1/HC−Pro-GFP^NIb/CP (a) or SCMV-GFP^P1/HC−Pro-mCherry^NIb/CP (b) were amplified as controls. Levels of CP transcript (lowest panels) amplified from tested samples served as internal controls