Fig. 6
HbSAMS5 has a function in ethylene biosynthesis. a, b The HbSAMS5 silencing by dsRNA application inhibited ethylene and ACC accumulations. The ethylene and ACC contents were determined at 5 dpi using HPLC–MS/MS (FW: Fresh weight). Statistical differences are marked with asterisks compared to the samples of WT (n = 9 leaf discs from 3 independent experiments. ** P < 0.01, Student’s t-test). c DAB staining showed that HbSAMS5-GFP expression and ACC treatment for 48 h induced ROS accumulation in N. benthamiana. d, e HbSAMS5 expression in N. benthamiana strongly enhanced the increase of ethylene and ACC under the condition of flg22 or DC3000 treatments. Statistical differences are marked with asterisks compared to the samples of mock (n = 9 leaf discs from 3 independent experiments. ** P < 0.01, Student’s t-test). f, g Either Eae1-GFP or Eae1-GFP combined with HbAIG1 inhibited ethylene and ACC accumulation in N. benthamiana, which was induced by HbSAMS5-FLAG. Statistical differences are marked with asterisks (n = 9 leaf discs from three independent experiments. ** P < 0.01, one-way ANOVA)